Liang X, Dai Z, Liu W
[Simultaneous determination of 5 sedative hypnotics in human plasma by reversed phase high-performance liquid chromatography.] [English Abstract, Journal Article]
Zhong Nan Da Xue Xue Bao Yi Xue Ban 2009 Jul; 34(7):689-92.
Objective To determine diazepam, nitrazepam, oxazepam, estazolam, and alprazolam simultaneously in human plasma by reversed phase high-performance liquid chromatography (RP-HPLC). Methods Ten microliter carbamazepine (50 mg/L)as the internal standard was added into 1mL sample, which contained the 5 mixed sedative hypnotics as standard substance and human plasma as ground substance. They were extracted with acetoacetate from plasma samples, and then were dissolved by 100 muL mobile phase. The blood drug levels were analyzed by high performance liquid chromatograph with 20 muL sample injection on a chromatographic column C18(4.6 mm*250 mm)at 30 degree. The mobile phase consisted of methanol and water (65:35),and the flow rate was 1.0 mL/min.The ultraviolet detection wavelength was 230 nm. Results The linearity range of the 5 drugs was 5~1 200 mug/L(r>/=0.9966, P<0.05).The recovery rate was 95.5%~105.6%.The extraction recovery rate was more than 75%. The relative standard deviation (RSD) of intra-day and inter-day was less than 10% (n=5).Conclusion RP-HPLC method is convenient, accurate and sensitive for simultaneous determination of the concentration of diazepam, nitrazepam, oxazepam, estazolam, and alprazolam in human plasma.
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